The collection comprises almost 200 photographs, including copy prints, film, contact sheets, documentary photographs, and personal snap shots, all relating to the life and work of Georgia O'Keeffe. Many photographs are of O'Keeffe or her New Mexico properties.

Part of this collection was accessioned into the Museum's art collection, including fine art photography by such artists as Alfred Stieglitz, Ansel Adams, Laura Gilpin, Todd Webb, and Philip Halsman. Those photographs share an accession number scheme beginning with 2014.03.

The collection also contains a limited amount of correspondence, ephemera, clippings, popular prints, and one watercolor and graphite work by an unknown individual.